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MemGlow NR12S is a solvatochromic photostable plasma membrane-targeting dye. Upon binding with a plasma membrane in a predominant liquid ordered (LO) phase NR12S exhibits a 45-50 nm wavelength shift relative to liquid disordered (LD) phase enabling investigators to examine the nanoscale distribution of local chemical polarity in plasma membranes. NR12S can also be used to label the plasma membrane for conventional fluorescence microscopy imaging.

NR12S Membrane Polarity Probe Product Functions (MG08)

MemGlow NR12S is a solvatochromic photostable plasma membrane-targeting dye

Examine the nanoscale distribution of local chemical polarity in plasma membranes

NR12S can also be used to label the plasma membrane for conventional fluorescence microscopy imaging.

Figure 1. Diagram of NR12S’s lipid order-dependent emission.

Figure 2. Absorbance and excitation spectra of MemGlow NR12S diluted in DMSO to 4.6 μM. Absorbance peak detected at 550 nm with peak emission at 633 nm.

Figure 3. Widefield fluorescent imaging of live HeLa S1 cells labeled with 10 nM NR12S. HeLa S1 cells were imaged with a TRITC filter set, neutral density filter, a digital CCD camera, and 100x oil objective (false colored red).